Hop bitter acids inhibit carbohydrate metabolism,enhance biogenic amine metabolism and alter L-malic acid,glutamic acid and arginine metabolism of Lactobacillus brevis 49

Beer contains only limited amounts of readily fermentable carbohydrates and amino acids. Beer spoilage lactic acid bacteria have to come up with metabolic strategies in order to deal with selective nutrient content. The research was performed to investigate the influence of iso-α-acids on the metabolism of organic acids, biogenic amines (BAs), off-flavour compounds, carbohydrates and amino acids of Lactobacillus brevis 49. Only glyoxylic acid and ethyl formate in de man, rogosa, sharpe broth was consumed, and multiple organic acids, BAs and off-flavour compounds were produced. By supplementing a series of concentrations of hop iso-α-acids, consumption of L-malic acid, glutamic acid and arginine and generation of BAs were found benefit for bacteria to develop hop-resistance. In the metabolism of carbohydrates, glucose was preferred over maltose and maltriose, and hops significantly inhibited the utilisation of carbohydrates. The results provide comprehensive information of metabolites of L. brevis 49 under various concentrations of hop stress.     

New approach to mimic rheological actual shear rate under wall slip condition

Engineering with Computers - The presence of wall slip in concentrated suspensions affect the rheological measurements such as shear stress, shear rate, and viscosity. The measured shear rate will...     

Robust Tracking Control with Preview Action for Uncertain Discrete-time Systems

International Journal of Control, Automation and Systems - This paper discusses the robust preview control problem for uncertain discrete-time systems, where future reference and disturbance...     

General Decay Synchronization and H ∞ Synchronization of Multi-weighted Coupled Reaction-diffusion Neural Networks

This paper investigates the general decay synchronization and general decay H∞ synchronization problems of multi-weighted coupled reaction-diffusion     

Investigation of machining and wear performance of various diamond micro-grinding tools

The International Journal of Advanced Manufacturing Technology - The wear of micro-grinding tools less than 1 mm in diameter is crucial for achieving acceptable surface quality and accurate...     

核电厂主螺栓超声检测与信号分析

依据RSE-M标准需要定期对核电厂反应堆压力容器（RPV）主螺栓进行超声检测，为了保证主螺栓螺纹区及光杆区不同深度刻槽的超声检测灵敏度，本文对检测工艺进行声场仿真计算，分析与判断数据采集中的相关信号与非相关信号，并重点分析裂纹信号的特征，验证了超声工艺的可靠性。结合现场实施案例，通过45°横波端角反射率高的特性，综合其他检测方法如涡流和渗透检测对缺陷性质进行判定，可有效确定异常信号。      

不同苯乙烯工艺技术经济分析及建议

通过对国内外苯乙烯市场供需趋势的梳理,针对4种苯乙烯主要生产工艺,重点从原料可获性、产品质量、能耗物耗以及成本竞争力等方面对不同工艺主要技术经济指标进行了对比分析,并提出了行业可持续发展的有关建议。     

非连续采空区矿柱力学分析的数值模拟

为探讨非连续采空区矿柱力学原理，通过FLAC3D数值模拟技术，对下向水平分层进路式胶结充填采矿法开采形成的非连续采空区中矿柱的受力状态进行了分析。研究表明，非连续采空区中矿柱所受的力主要为覆岩的自重应力，水平应力对其影响不大；随着埋深的增加矿柱承受的垂直方向的力近似呈直线增加；随着非连续采空区跨度的增加（进路数量增加），非连续采空区受垂直方向的应力逐渐由围岩两侧向矿柱中间过渡。     

Aptamer/Peptide-Functionalized Genome-Editing System for Effective Immune Restoration through Reversal of PD-L1-Mediated Cancer Immunosuppression

Effective reversal of tumor immunosuppression is of critical importance in cancer therapy. A multifunctional delivery vector that can effectively deliver CRISPR-Cas9 plasmid for β-catenin knockout to reverse tumor immunosuppression is constructed. The multi-functionalized delivery vector is decorated with aptamer-conjugated hyaluronic acid and peptide-conjugated hyaluronic acid to combine the tumor cell/nuclear targeting function of AS1411 with the cell penetrating/nuclear translocation function of TAT-NLS. Due to the significantly enhanced plasmid enrichment in malignant cell nuclei, the genome editing system can induce effective β-catenin knockout and suppress Wnt/β-catenin pathway, resulting in notably downregulated proteins involved in tumor progression and immunosuppression. Programmed death-ligand 1 (PD-L1) downregulation in edited tumor cells not only releases the PD-1/PD-L1 brake to improve the cancer killing capability of CD8⁺ T cells, but also enhances antitumor immune responses of immune cells. This provides a facile strategy to reverse tumor immunosuppression and to restore immunosurveillance and activate anti-tumor immunity.